Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free
    See all Integrin alpha 4/CD49D primary antibodies

  • Description

    Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab75760 only detects Integrin alpha 4/CD49D phosphorylated on Serine 988 of the mature protein. S988 coresponds to S1021 of the sequence with the 33 aa signal peptide and S1027 of the precursor shown in Figure 4 of PMID: 8436405.

    The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa).

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247393 is the carrier-free version of ab75760 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247393 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Integrin alpha 4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
    Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
    All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/1000 dilution (purified)

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 150 kDa
    Observed band size: 150,70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab75760, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
    Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
    All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/5000 dilution (purified)

    Lane 1 : untreated Jurkat lysate
    Lane 2 : Jurkat cell lysate treated with Lambda phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 150 kDa
    Observed band size: 150 kDa



    This data was developed using ab75760, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
    Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
    All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/2000 dilution

    Lane 1 : THP1 cell lysates, untreated
    Lane 2 : THP1 cell lysates, with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 150 kDa
    Observed band size: 140,180,70 kDa why is the actual band size different from the predicted?



    This data was developed using ab75760, the same antibody clone in a different buffer formulation.

     

  • Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
    Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)

© 2021 Astana Biomed Group LLP. All rights reserved.