Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
![Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)](https://www.abcam.com/ps/products/247/ab247393/Images/ab247393-406323-anti-integrin-alpha-4-cd49d-phospho-s988-antibody-epr2343-bsa-and-azide-free-western-blot.jpg "Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free
See all Integrin alpha 4/CD49D primary antibodies -
Description
Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988) - BSA and Azide free -
Host species
Rabbit -
Specificity
ab75760 only detects Integrin alpha 4/CD49D phosphorylated on Serine 988 of the mature protein. S988 coresponds to S1021 of the sequence with the 33 aa signal peptide and S1027 of the precursor shown in Figure 4 of PMID: 8436405.
The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa).
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247393 is the carrier-free version of ab75760 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247393 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Integrin alpha 4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2343 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/1000 dilution (purified)
Lane 1 : Rat spleen tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 150 kDa
Observed band size: 150,70 kDa why is the actual band size different from the predicted?This data was developed using ab75760, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)](https://www.abcam.com/ps/products/247/ab247393/Images/ab247393-403249-anti-integrin-alpha-4-cd49d-phospho-s988-antibody-epr2343-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/5000 dilution (purified)
Lane 1 : untreated Jurkat lysate
Lane 2 : Jurkat cell lysate treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDaThis data was developed using ab75760, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)](https://www.abcam.com/ps/products/247/ab247393/Images/ab247393-399267-anti-integrin-alpha-4-cd49d-phospho-s988-antibody-epr2343-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/2000 dilution
Lane 1 : THP1 cell lysates, untreated
Lane 2 : THP1 cell lysates, with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 150 kDa
Observed band size: 140,180,70 kDa why is the actual band size different from the predicted?This data was developed using ab75760, the same antibody clone in a different buffer formulation.
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![Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)](https://www.abcam.com/ps/products/247/ab247393/Images/ab247393-4-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] - BSA and Azide free (ab247393)
