Anti-AMSH antibody [EPR4361] (ab108301)
![Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-387259-anti-stambp-antibody-western-blot-hela-hap1-jurkat-lysates.jpg "Anti-AMSH antibody [EPR4361] (ab108301)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4361] to AMSH
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-AMSH antibody [EPR4361]
See all AMSH primary antibodies -
Description
Rabbit monoclonal [EPR4361] to AMSH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat cell lysate, HeLa cell lysate, HAP1 cell lysate, Raji cell lysate, SW480 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4361 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMSH antibody [EPR4361] (ab108301)All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : STAM Binding Protein knockout HeLa lysate
Lane 3 : HAP1 lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDaLanes 1-4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control ab8245 observed at 37 kDa.
ab108301 Anti-STAMBP antibody was shown to specifically react with STAM Binding Protein in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265249 (knockout cell lysate ab258213) was used. Wild-type and STAM Binding Protein knockout samples were subjected to SDS-PAGE. ab108301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-281802-anti-amsh-antibody-epr4361-western-blot.jpg)
Western blot - Anti-AMSH antibody [EPR4361] (ab108301)All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : AMSH knockout HAP1 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48 kDaLanes 1 - 4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108301 was shown to specifically react with AMSH in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when AMSH knockout samples were examined. Wild-type and AMSH knockout samples were subjected to SDS-PAGE. Ab108301 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-120817-anti-amsh-antibody-epr4361-western-blot.jpg)
Western blot - Anti-AMSH antibody [EPR4361] (ab108301)All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDa
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![Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-4-benefits-of-recombinant-antibodies.png)
Anti-AMSH antibody [EPR4361] (ab108301)
