Anti-DGKE antibody [EPR7847] (ab128949)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7847] to DGKE
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-DGKE antibody [EPR7847]
See all DGKE primary antibodies -
Description
Rabbit monoclonal [EPR7847] to DGKE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
A synthetic peptide corresponding to residues in Human DGKE (UniProt P52429).
-
Positive control
- 293T, HeLa and K562 cell lysates
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.09 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7847 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-DGKE antibody [EPR7847] (ab128949) at 1/1000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates prepared in RIPA lysis method
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
-
All lanes : Anti-DGKE antibody [EPR7847] (ab128949) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Hela cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaThe cell lysates were prepared in 1%SDS Hot lysis method.
-