Anti-Sonic Hedgehog antibody (ab240438)
Key features and details
- Goat polyclonal to Sonic Hedgehog
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Sonic Hedgehog antibody
See all Sonic Hedgehog primary antibodies -
Description
Goat polyclonal to Sonic Hedgehog -
Host species
Goat -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Sonic Hedgehog aa 400 to the C-terminus (C terminal) (Cysteine residue). (NP_000184.1)
Database link: Q15465 -
Positive control
- WB: Human liver lysate. ICC/IF: HeLa, U-251 MG and NIH/3T3 cells. Flow Cyt: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sonic Hedgehog antibody (ab240438)Anti-Sonic Hedgehog antibody (ab240438) at 2 µg/ml + Human liver tissue lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
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Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (ab240438)Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Sonic Hedgehog (Green) using ab240438 in ICC/IF. Primary incubation for 1 hour (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (Blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Flow Cytometry - Anti-Sonic Hedgehog antibody (ab240438)Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) labeling Sonic Hedgehog (blue line) with ab240438. Primary incubation for 1 hour at 10 μg/ml, followed by Alexa Fluor®488 secondary antibody (1 μg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor®488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (ab240438)Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized U-251 MG (human brain glioma cell line) cells labeling Sonic Hedgehog (Green) using ab240438 in ICC/IF. Primary incubation for 1 hour (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (ab240438)Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Sonic Hedgehog (Green) using ab240438 in ICC/IF. Primary incubation for 1 hour (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (Blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
