Anti-GSK3 alpha antibody (ab78664)
Key features and details
- Rabbit polyclonal to GSK3 alpha
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GSK3 alpha antibody
See all GSK3 alpha primary antibodies -
Description
Rabbit polyclonal to GSK3 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human GSK3 alpha aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93065) -
Positive control
- WB:HeLa; A549; HEK293 and HepG2 cell lysates; Mouse and Rat Brain Tissue Lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications CowApplication Abreviews Notes WB 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).Notes WB
1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).Target
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Function
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 536561 Cow
- Entrez Gene: 2931 Human
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Omim: 606784 Human
- SwissProt: P49840 Human
- SwissProt: Q2NL51 Mouse
- SwissProt: P18265 Rat
see all -
Alternative names
- DKFZp686D0638 antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase-3 alpha antibody
see all
Images
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Western blot - Anti-GSK3 alpha antibody (ab78664)All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : GSK3A knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab78664 observed at 51 kDa. Red - loading control ab8245 observed at 36 kDa.
ab78664 Anti-GSK3 alpha antibody was shown to specifically react with GSK3 alpha in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266742 (knockout cell lysate ab257979) was used. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab78664 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-GSK3 alpha antibody (ab78664)Lane 1: GSK3A knockout HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab78664 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab78664 was shown to recognize GSK3A when GSK3A knockout samples were used, along with additional cross-reactive bands. Wild-type and GSK3A knockout samples were subjected to SDS-PAGE. Followed by incubation with ab78664 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a 1/250 and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-GSK3 alpha antibody (ab78664)All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes -
Western blot - Anti-GSK3 alpha antibody (ab78664)All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab78664 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-GSK3 alpha antibody (ab78664)All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : GSK3A knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab78664 observed at 51 kDa. Red - loading control ab8245 observed at 36 kDa.
ab78664 Anti-GSK3 alpha antibody was shown to specifically react with GSK3 alpha in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266742 (knockout cell lysate ab257979) was used. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab78664 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-GSK3 alpha antibody (ab78664)
Lane 1: GSK3A knockout HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab78664 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab78664 was shown to recognize GSK3A when GSK3A knockout samples were used, along with additional cross-reactive bands. Wild-type and GSK3A knockout samples were subjected to SDS-PAGE. Followed by incubation with ab78664 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a 1/250 and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-GSK3 alpha antibody (ab78664)All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
-
Western blot - Anti-GSK3 alpha antibody (ab78664)All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
