Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GGA1 antibody [EPR3014(2)] (ab170956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3014(2)] to GGA1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GGA1 antibody [EPR3014(2)]
    See all GGA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3014(2)] to GGA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GGA1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UJY5

  • Positive control

    • HeLa, Jurkat, 293T and K562 cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3014(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
    Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GGA1 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab170956 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab170956 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab170956 and ab8245 (loading control to GAPDH) were diluted 2 µg/ml and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
    Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956)

    Western blot analysis on Immunoprecipitation pellet from Jurkat cells showing GGA1, using ab170956 at 1/10 dilution for IP.

  • Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
    Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
    All lanes : Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa

  • Anti-GGA1 antibody [EPR3014(2)] (ab170956)
    Anti-GGA1 antibody [EPR3014(2)] (ab170956)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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