Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GGA1 antibody [EPR3014(2)] (ab170956)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3014(2)] to GGA1
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-GGA1 antibody [EPR3014(2)]
See all GGA1 primary antibodies
Description

Rabbit monoclonal [EPR3014(2)] to GGA1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human GGA1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UJY5
Positive control
- HeLa, Jurkat, 293T and K562 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3014(2)
Isotype

IgG
Research areas

Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GGA1 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab170956 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170956 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab170956 and ab8245 (loading control to GAPDH) were diluted 2 µg/ml and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956)

Western blot analysis on Immunoprecipitation pellet from Jurkat cells showing GGA1, using ab170956 at 1/10 dilution for IP.
Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)

All lanes : Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 70 kDa
Anti-GGA1 antibody [EPR3014(2)] (ab170956)

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