Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CHMP2B antibody [EPR10807(B)] (ab157208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10807(B)] to CHMP2B
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CHMP2B antibody [EPR10807(B)]
    See all CHMP2B primary antibodies

  • Description

    Rabbit monoclonal [EPR10807(B)] to CHMP2B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human CHMP2B (Uniprot: Q9UQN3)

  • Positive control

    • Recombinant Human CHMP2B protein (ab109959) can be used as a positive control in WB. HepG2, A431 and 293T cell lysates; fetal brain lysate; HepG2 and 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : CHMP2B knockout A549 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 24 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab157208 observed at 45 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab157208 was shown to specifically react with CHMP2B in wild-type A549 cells as signal was lost in CHMP2B knockout cells. Wild-type and CHMP2B knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab157208 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Immunocytochemistry/ Immunofluorescence - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Immunofluorescence analysis of HepG2 cells, labeling CHMP2B using ab157208 at a 1/250 dilution.
  • Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 24 kDa

  • Immunoprecipitation - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Immunoprecipitation - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing CHMP2B, using ab157208 at 1/10 dilution for IP
  • Flow Cytometry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Flow Cytometry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Flow cytometry analysis of permeabilized 293T cells labeling CHMP2B (red), using ab157208 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
  • Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
    Anti-CHMP2B antibody [EPR10807(B)] (ab157208)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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