Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

Order now and get it on Thursday March 04, 2021

Anti-CLCA1 antibody [EPR12254] (ab174319)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12254] to CLCA1
Suitable for: IHC-P, WB, IP
Reacts with: Human

Product name

Anti-CLCA1 antibody [EPR12254]
See all CLCA1 primary antibodies
Description

Rabbit monoclonal [EPR12254] to CLCA1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CLCA1.
Database link: A8K7I4
Positive control
- Human colon cancer and Human small intestine lysates; Human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12254
Isotype

IgG
Research areas

Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)

All lanes : Anti-CLCA1 antibody [EPR12254] (ab174319) at 1/10000 dilution

Lane 1 : Human colon cancer lysate
Lane 2 : Human small intestine lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 100 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLCA1 with ab174319 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)

Western blot analysis on immunoprecipitation pellet from Human colon cancer lysate using ab174319 at 1/10 dilution.
Anti-CLCA1 antibody [EPR12254] (ab174319)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com