Antibodies, Proteins, Kits and Reagents for Life Science

HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR12254-88] to CLCA1
Suitable for: WB
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-CLCA1 antibody [EPR12254-88]
See all CLCA1 primary antibodies
Description

HRP Rabbit monoclonal [EPR12254-88] to CLCA1
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
Database link: A8K7I4
Positive control
- WB: Small Intestine (Human) Tissue Lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12254-88
Isotype

IgG
Research areas

Western blot - HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)

HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736) at 1/1000 dilution + Small Intestine (Human) Tissue Lysate at 10 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 100 kDa
Observed band size: 85 kDa
why is the actual band size different from the predicted?

Exposure time: 16 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203736 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)

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