Formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Estrogen Receptor alpha using ab259427 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

MCF7 (human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha using ab259427 at 2 μg/ml (green) in ICC/IF.

The membrane is stained with Phalloidin (red).

Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha using ab259427 at 2 μg / 10⁶ cells (blue) as compared to an isotype control (red).

The secondary antibody is a Goat anti-Mouse IgG CF488 conjugate.

Protein array containing >19,000 full-length human proteins, probed with ab259427.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Estrogen Receptor alpha using ab259427 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.