Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4097] to Estrogen Receptor alpha
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Estrogen Receptor alpha antibody [EPR4097]
See all Estrogen Receptor alpha primary antibodies
Description

Rabbit monoclonal [EPR4097] to Estrogen Receptor alpha
Host species

Rabbit
Specificity

Expression levels of ER alpha protein vary with sample type.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7 and T47-D cell lysates. IHC-P: Human breast ductal infiltrating carcinoma and normal breast tissues. ICC/IF: MCF-7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4097
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling Estrogen Receptor alpha with purified ab108398 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

All lanes : Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398) at 1/2000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
Lane 2 : T-47D (human mammary gland ductal carcinoma epithelial cell). Whole cell lysates
Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 5 : Human uterus whole tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 68 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398) at 1/1000 dilution + GST-Tagged Recombinant Human Estrogen Receptor beta (aa 1 to 323) protein 100ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 66 kDa

Exposure time: 180 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Lane 1: Recombinant Human Estrogen Receptor beta (aa 1 to 323) protein, (Cat#: ab158385)
There is no cross-reactivity between ab108398 and ER beta protein.
Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

All lanes : purified at 1/2000 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : T47-D cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 66 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Immunocytochemsitry/Immunofluorescence analysis of MCF-7 cells labelling Estrogen Receptor alpha (green) with purified ab108398 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Flow Cytometry - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

ab108398 staining Estrogen Receptor alpha in the human cell line MCF-7(human breast carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast ductal infiltrating carcinoma tissue labelling Estrogen Receptor alpha with unpurified ab108398.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human normal tonsil tissue. Unpurified ab108398 shows negative staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human colonic adenocarcinoma tissue. Unpurified ab108398 shows negative staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human lung adenocarcinoma tissue. Unpurified ab108398 shows negative staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human cervical carcinoma tissue. Unpurified ab108398 shows negative staining.
Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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