Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S167)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Estrogen Receptor alpha (phospho S167) antibody
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor alpha (phospho S167) -
Host species
Rabbit -
Specificity
ab31478 detects endogenous levels of estrogen receptor alpha only when phosphorylated at Serine 167. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic phosphopeptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Serine 167
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ab31478 at 1/50. Left: Untreated; Right: Treated with synthesized phosphopeptide.
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Western blot - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)All lanes : Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478) at 1/500 dilution
Lane 1 : HeLa cells treated with EGF
Lane 2 : Untreated HeLa cell lysate
Predicted band size: 68 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478) This image is courtesy of an anonymous Abreviewab31478 staining human non-small cell lung cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in 10 mM sodium citrate (pH 6) prior to blocking with a commercially available blocking reagent for 20 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 24 hours at 4°C. A HRP conjugated antibody was used as the secondary.
