Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR703(2)] to Estrogen Receptor alpha
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Estrogen Receptor alpha antibody [EPR703(2)]
    See all Estrogen Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR703(2)] to Estrogen Receptor alpha

  • Host species

    Rabbit

  • Specificity

    Expression levels of ER alpha protein vary with sample type.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Estrogen Receptor alpha aa 550-650 (C terminal). The exact sequence is proprietary.

  • Positive control

    • MCF-7 cell lysate. Human breast carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
    Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
    All lanes : Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413) at 1/2000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
    Lane 2 : T-47D (human mammary gland ductal carcinoma epithelial cell). Whole cell lysates
    Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (Negative control)
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) Whole cell lysates (Negative control)
    Lane 5 : Human uterus whole tissue lysate
    Lane 6 : Human ovary whole tissue lysate
    Lane 7 : Human ovary cancer whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
    Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
    Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413) at 1/1000 dilution + MCF-7 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 68 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

    ab79413, at 1/500 dilution, staining Estrogen Receptor alpha in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413) This image is courtesy of an Abreview submitted by Marcia Burch

    ab79413 staining Estrogen Receptor alpha in Baboon placenta and fetal ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS + 5% normal goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (7µg/ml) was used as the secondary antibody.

    See Abreview

  • Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
    Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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