Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E91] to Estrogen Receptor alpha (phospho S118) - BSA and Azide free
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free
    See all Estrogen Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [E91] to Estrogen Receptor alpha (phospho S118) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Dot blotmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab177817 is the carrier-free version of ab32396. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab177817 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) treated with EGF (100ng/ml, 5min) and treated with Lambda Protein Phosphatase 31? for 2h cells labeling Estrogen receptor alpha (phospho S118) with purified ab32396 at 1:200 dilution (8.9μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
    Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 5 μg/ml (1/200 dilution). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an AlexaFluor®488 Goat anti-Rabbit  was used as the secondary antibody at 2 μg/ml (1/1000 dilution). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 2.5 μg/ml (1/200 dilution). DAPI nuclear counterstain.
    Confocal image showing the signal increased after EGF (100ng/ml, 5 min) treatment and decreased after Lambda Protein Phosphatase treatment (31°C for 2 hours).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

  • Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
    Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

    Dot blot analysis of Lane 1: Estrogen Receptor alpha (pS118) phospho peptide and Lane 2: Estrogen Receptor alpha non-phospho peptide labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
    Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

    Immunofluorescent staining of (A) untreated and (B) Phosphatase-treated MCF-7 cells using unpurified ab32396.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

    Immunohistochemical staining of (A) untreated and (B) Phosphatase-treated paraffin-embedded human breast adenocarcinoma using unpurified ab32396 at 1:50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

  • Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
    Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

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