Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37] - BSA and Azide free (ab242439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BUI-1-37] to Estrogen Receptor alpha (phospho S294) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37] - BSA and Azide free
    See all Estrogen Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [BUI-1-37] to Estrogen Receptor alpha (phospho S294) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with myc-his tagged ER alpha overexpression vector whole cell lysate; HEK-293T transfected with myc-his tagged ER alpha overexpression vector treated with 10nM E2 for 25min whole cell lysate; HEK-293T transfected with myc-his tagged ER alpha overexpression vector treated with 1uM Dinaciclib (CDK2 inhibitor) for 1h, then add 10nM E2 for 25min whole cell lysate.

  • General notes

    ab242439 is a PBS-only buffer format of ab207602. Please refer to ab207602 for recommended dilutions, protocols, and image data.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Western blot protocol advice:

    Since intracellular levels of pS294 ER are very low, Western blot without prior immunoprecipitation of total ER is not sensitive enough to detect pS294 ER. We recommend initial ER immunoprecipitation, following which it is possible to use this antibody for Western blot (see data image).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37] - BSA and Azide free (ab242439)
    Western blot - Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37] - BSA and Azide free (ab242439)
    All lanes : Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37] (ab207602) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HEK-293T transfected with myc-His tagged ER alpha overexpression vector whole cell lysate
    Lane 3 : HEK-293T transfected with myc-His tagged ER alpha overexpression vector treated with 10nM E2 for 25min whole cell lysate
    Lane 4 : HEK-293T transfected with myc-His tagged ER alpha overexpression vector treated with 1uM Dinaciclib (CDK2 inhibitor) for 1h, then add 10nM E2 for 25min whole cell lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 67 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Harvested cells were lysed and immunoprecipitated using an ERα antibody; and the immunoprecipitated ERα (20μl each from un-transfected, ER alpha vector transfected, E2 and Din/E2 samples) was used to prepare separate western blots probed with either ab207602 anti-ERpS294 monoclonal or total ERα, all of which run at 67 kDa molecular weight. (PMID: 29137354).

    Blocking/Dilution bufer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, arginine and sodium azide (ab207602).

  • Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37] - BSA and Azide free (ab242439)
    Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37] - BSA and Azide free (ab242439)

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