Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR034F] to FOXP3 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-FOXP3 antibody [BLR034F] - BSA free
See all FOXP3 primary antibodies -
Description
Rabbit monoclonal [BLR034F] to FOXP3 - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human FOXP3 aa 275-325. The exact sequence is proprietary. NP_054728.2 and Gene ID 50943.
Database link: Q9BZS1 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR034F -
Isotype
IgG -
Research areas
Images
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FOXP3 was immunoprecipitated from 1.0 mg MJ whole cell lysate with ab243898 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243898 at 1/1000 dilution.
Lane 1: ab243864 IP in HeLa cell lysate.
Lane 2: Contol IgG in MJ whole cell lysate.
Detection: Chemiluminescence with an exposure time of 75 seconds.
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Western blot analysis using ab243890 at 1/1000 dilution.
Lane 1: MJ whole cell lysate (50 µg).
Lane 2: Jurkat whole cell lysate (50 µg).
Lane 3: HEK-293T whole cell lysate (50 µg).
Lane 4: MOLT-4 whole cell lysate (50 µg).
Lane 5: RPMI 8226 whole cell lysate (50 µg).
Lane 6: HH whole cell lysate (50 µg).
Lane 7: C5/MJ whole cell lysate (50 µg).
Lane 8: KG-1 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 minutes.
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Formalin-fixed, paraffin-embedded MJ cells labeling FOXP3 using ab243890 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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