Anti-TRP2/DCT antibody [EPR21986] (ab221144)
![Anti-TRP2/DCT antibody [EPR21986] (ab221144)](https://www.abcam.com/ps/products/221/ab221144/Images/ab221144-309268-anti-trp2-dct-antibody-epr21986-western-blot.jpg "Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21986] to TRP2/DCT
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TRP2/DCT antibody [EPR21986]
See all TRP2/DCT primary antibodies -
Description
Rabbit monoclonal [EPR21986] to TRP2/DCT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP MouseHumanWB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MeWo, SK-MEL-2, SK-MEL-28 and F9 whole cell lysates. IHC-P: Human skin and melanoma tissues. IP: SK-MEL-2 and B16-F0 whole cell lysates.
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General notes
Previously labelled as TRP2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21986 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRP2/DCT antibody [EPR21986] (ab221144)All lanes : Anti-TRP2/DCT antibody [EPR21986] (ab221144) at 1/1000 dilution
Lane 1 : MeWo (human malignant melanoma cell line) whole cell lysate
Lane 2 : SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate
Lane 3 : SK-MEL-28 (human malignant melanoma cell line) whole cell lysate
Lane 4 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 69,85 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time : Lane 1: 5 seconds; Lane 2: 15 seconds; Lane 3: 26 seconds; Lane 4: 3 minutes.
The molecular mass observed is consistent with what has been described in the literature (PMID: 12719423, PMID: 25009701)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)](https://www.abcam.com/ps/products/221/ab221144/Images/ab221144-309270-anti-trp2-dct-antibody-epr21986-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human melanoma (PMID: 24475287). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)](https://www.abcam.com/ps/products/221/ab221144/Images/ab221144-309272-anti-trp2-dct-antibody-epr21986-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)TRP2/DCT was immunoprecipitated from 0.35 mg B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: B16-F0 whole cell lysate 10 µg (Input).
Lane 2: ab221144 IP in B16-F0 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221144 in B16-F0 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
~85KD band is the glycosylated form (PMID: 12719423)
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![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)](https://www.abcam.com/ps/products/221/ab221144/Images/ab221144-309271-anti-trp2-dct-antibody-epr21986-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)TRP2/DCT was immunoprecipitated from 0.35 mg SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: SK-MEL-2 whole cell lysate 10 µg (Input).
Lane 2: ab221144 IP in SK-MEL-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221144 in SK-MEL-2 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)](https://www.abcam.com/ps/products/221/ab221144/Images/ab221144-309269-anti-trp2-dct-antibody-epr21986-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in melanocytes of human skin (PMID:12535195). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-TRP2/DCT antibody [EPR21986] (ab221144)](https://www.abcam.com/ps/products/221/ab221144/Images/ab221144-6-benefits-of-recombinant-antibodies.png)
Anti-TRP2/DCT antibody [EPR21986] (ab221144)
