Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

Key features and details

  • Mouse monoclonal [mAbcam 22510] to FOXP3 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free
    See all FOXP3 primary antibodies

  • Description

    Mouse monoclonal [mAbcam 22510] to FOXP3 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    Detects a single clean band on human FOXP3 transfected 293 cells. Gives a very similar staining pattern to the ab20034 on human tonsil, confirming its specificity for FOXP3. Detects FOXP3 in mouse samples in western blot. However, not suitable for mouse in IHC.

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and spleen tissue. Human colon carcinoma tissue. IHC-Fr: Human tonsil tissue. WB: HEK293 cells overexpressing human FOXP3.

  • General notes

    ab188638 is the PBS only version of ab22510.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

    Image produced using the same antibody clone but in a different formulation ab22510.

    IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
    Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

    Image produced using the same antibody clone but in a different formulation ab22510.

    IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

    Image produced using the same antibody clone but in a different formulation ab22510.

    IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human spleen* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

    Image produced using the same antibody clone but in a different formulation ab22510.

    IHC image of ab22510 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22510, 20µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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