Anti-FOXP3 antibody [SP97] (ab99963)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP97] to FOXP3
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-FOXP3 antibody [SP97]
See all FOXP3 primary antibodies -
Description
Rabbit monoclonal [SP97] to FOXP3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, pancreatic adenocarcinoma, stomach adenocarcinoma, lymph node, B cell lymphoma, colon, bladder, bladder transitional cell carcinoma, colon adenocarcinoma, thymus and cervical squamous cell carcinoma.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP97 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP3 with ab99963, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on a subset of T cells in the human tonsil. The section was incubated with ab229902 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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Immunohistochemical analysis of Human Tonsil tissue labelling FOXP3 with ab99963.
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Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lymph node tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human B cell lymphoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
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