Western blot protocol advice:

Since intracellular levels of pS294 ER are very low, Western blot without prior immunoprecipitation of total ER is not sensitive enough to detect pS294 ER. We recommend initial ER immunoprecipitation, following which it is possible to use this antibody for Western blot (see data image).

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com or you can find further information here.

This product has switched from a hybridoma to recombinant production method on 02^nd November 2020.

This product is a recombinant monoclonal antibody, which offers several advantages including:

• - High batch-to-batch consistency and reproducibility
• - Improved sensitivity and specificity
• - Long-term security of supply
• - Animal-free production

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.