Anti-Estrogen Receptor alpha antibody (ab3575)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha
- Suitable for: ICC, IHC-P, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Estrogen Receptor alpha antibody
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human, Recombinant fragment
Predicted to work with: Hamster, Cow, Cat
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Immunogen
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Positive control
- WB: recombinant fragment; IHC: rat prostate tissue; ICC: T47D and 578T breast cancer cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
IHC image of Estrogen Receptor alpha staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab3575, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.
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Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution
Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDaBlocked with 5% milk in TBST for at least 1 hour at room temperature.
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Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.
