Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12254-88] to CLCA1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free
    See all CLCA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12254-88] to CLCA1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
    Database link: A8K7I4

  • Positive control

    • IHC-P: Human small intestine tissue.

  • General notes

    Ab197059 is the carrier-free version of ab180851. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab197059 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)
    Flow Cytometry - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)
    Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

    Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)
    Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

    Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue sections labeling CLCA1 with purified ab180851 at 1:4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab180851).

  • Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)
    Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

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