Anti-DDX5 antibody [2257C3a] (ab53216)
![Anti-DDX5 antibody [2257C3a] (ab53216)](https://www.abcam.com/ps/products/53/ab53216/Images/ab53216-293569-anti-ddx5-antibody-2257c3a-western-blot.jpg "Anti-DDX5 antibody [2257C3a] (ab53216)")
Key features and details
- Mouse monoclonal [2257C3a] to DDX5
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-DDX5 antibody [2257C3a]
See all DDX5 primary antibodies -
Description
Mouse monoclonal [2257C3a] to DDX5 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment of human DDX5.
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Positive control
- HeLa whole cell lysate (ab150035), NIH3T3 whole cell lysate and F2408 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab53216 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
2257C3a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab53216 observed at 69 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab53216 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab53216 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)](https://www.abcam.com/ps/products/53/ab53216/Images/ab53216-65992-anti-ddx5-antibody-2257c3a-western-blot.gif)
Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + F2408 whole cell lysate at 25 µg
Secondary
anti-Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 69 kDa
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![Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)](https://www.abcam.com/ps/products/53/ab53216/Images/ab53216-65991-anti-ddx5-antibody-2257c3a-western-blot.gif)
Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
anti-Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 69 kDa
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![Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)](https://www.abcam.com/ps/products/53/ab53216/Images/ab53216-65990-anti-ddx5-antibody-2257c3a-western-blot.gif)
Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 69 kDa
