Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Thursday February 25, 2021

Key features and details

Rabbit polyclonal to Calcineurin A
Suitable for: WB
Reacts with: Mouse, Rat, Human, Recombinant fragment
Isotype: IgG

Product name

Anti-Calcineurin A antibody
See all Calcineurin A primary antibodies
Description

Rabbit polyclonal to Calcineurin A
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human, Recombinant fragment
Immunogen

Full length Calcineurin A fusion expressed in E. coli.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Concentration information loading...
Purity

Whole antiserum
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-Calcineurin A antibody (ab3673)

All lanes : Anti-Calcineurin A antibody (ab3673) at 1/200 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Observed band size: 62 kDa
why is the actual band size different from the predicted?
Western blot - Anti-Calcineurin A antibody (ab3673)

Western Blot of wild type mouse brain tissue homogenate with ab3673 used at 1/6000. Please note that this image was assembled from two different blots. (The numbers on the scale correspond to kD).
Western blot - Anti-Calcineurin A antibody (ab3673) This image is a courtesy of Anonymous Abreview

Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Huh7 (Human hepatocellular carcinoma) whole cell lysate at 15000 cells

Secondary
HRP conjugated sheep polyclonal to rabbit IgG at 1/15000 dilution

Performed under reducing conditions.

Observed band size: 62 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute
See Abreview
Western blot - Anti-Calcineurin A antibody (ab3673)

Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Recombinant Human Calcineurin A protein (ab101870) at 0.1 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Exposure time: 10 seconds

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