Bradykinin ELISA kit (ab136936)
Key features and details
- Sensitivity: 24.8 pg/ml
- Range: 11.7 pg/ml - 30000 pg/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
Overview
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Product name
Bradykinin ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Buffer 20 695.4pg/ml 4.6% Buffer 20 208.7pg/ml 6.2% Buffer 20 73.7pg/ml 9.9% Inter-assay Sample n Mean SD CV% Buffer 700.4pg/ml = 15% Buffer 209.3pg/ml = 10.3% Buffer 66.1pg/ml = 11.9% -
Sample type
Urine, Serum, Plasma -
Assay type
Competitive -
Sensitivity
24.8 pg/ml -
Range
11.7 pg/ml - 30000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 109 10pg/ml - 2000pg/ml Serum 113 100pg/ml - 2000pg/ml Plasma 110 100pg/ml - 20000pg/ml -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Species independent -
Product overview
Bradykinin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the accurate quantitative measurement of Bradykinin in plasma, serum and urine.
A goat anti-Rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of Bradykinin conjugated to biotin, followed by a solution of polyclonal antibody to Bradykinin. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of Bradykinin captured in the plate.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 16 1 x 55ml Bradykinin Antibody 1 x 5ml Bradykinin Conjugate 1 x 5ml Bradykinin Standard 2 vials Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit HRP- Streptavidin Conjugate 1 x 12.5µg Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 2 x 10ml -
Research areas
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Function
(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. -
Tissue specificity
Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors. -
Involvement in disease
Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. -
Sequence similarities
Contains 3 cystatin domains. -
Post-translational
modificationsBradykinin is released from kininogen by plasma kallikrein.
Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
Phosphorylation sites are present in the extracelllular medium.
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- Alpha-2-thiol proteinase inhibitor
- BDK
- Fitzgerald factor
see all
Images
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Typical Standard Curve
Representative standard curve using ab136936.