Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12254-88] to CLCA1
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CLCA1 antibody [EPR12254-88]
    See all CLCA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12254-88] to CLCA1

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
    Database link: A8K7I4

  • Positive control

    • Human colon cancer and human small intestine lysates; human colon tissue; A431 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/10000 dilution (purified) + Human small intestine lysates at 15 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 100 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Flow Cytometry - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

    Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue sections labeling CLCA1 with Purified ab180851 at 1:4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/50000 dilution (purified)

    Lane 1 : Rat small intestine lysates
    Lane 2 : Mouse small intestine lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

    Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.

  • Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/1000 dilution (unpurified)

    Lane 1 : Human colon cancer lysates
    Lane 2 : Human small intestine lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 100 kDa

  • Anti-CLCA1 antibody [EPR12254-88] (ab180851)
    Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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