Anti-Nogo A antibody (ab62024)
Key features and details
- Rabbit polyclonal to Nogo A
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nogo A antibody -
Description
Rabbit polyclonal to Nogo A -
Host species
Rabbit -
Specificity
ab62024 is specific for Nogo A and Nogo E. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Raised against a 19 amino acid synthetic peptide from near the centre of Human Nogo A (GenBank accession no. NP_065393).
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Positive control
- WB: Human brain tissue lysate. IHC-P: Mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nogo A antibody (ab62024)Lane 1 : Anti-Nogo A antibody (ab62024) at 0.5 µg/ml
Lane 2 : Anti-Nogo A antibody (ab62024) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG
Predicted band size: 130 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 155 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo A antibody (ab62024)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nogo A with Anti-Nogo A antibody (ab62024) at 5μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo A antibody (ab62024)ab62024, at 2.5µg/ml, staining Mouse Nogo A in brain tissue, by Immunohistochemistry -
Immunocytochemistry/ Immunofluorescence - Anti-Nogo A antibody (ab62024)ICC/IF image of ab62024 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62024, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-Nogo A antibody (ab62024)Immunofluorescence of NogoA in Mouse Brain cells using ab62024 at 20 ug/ml.
