Recombinant Human PAG protein (ab56521)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB
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Product name
Recombinant Human PAG protein -
Purity
> 95 % SDS-PAGE.
Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. > 95% (by SDS-PAGE) -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
41 to 432 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.4
Constituents: 0.164% Sodium phosphate, 0.58% Sodium chloride -
ReconstitutionReconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
Images
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SDS-PAGE - Recombinant Human PAG protein (ab56521)SDS PAGE analysis of ab56521: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1-5: PAG recombinant protein (2 µg, 1 µg, 0.5 µg, 0.2 µg, 0.1 µg per lane).
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Western blot - Recombinant Human PAG protein (ab56521)Western Blot analysis of ab56521. Reducing conditions. Lane 1-4: immunostaining with specific anti PAG antibody. Detection with Goat anti-rabbit / HRP secondary antibody. Amount of PAG recombinant protein loaded on each lane: (1) 0.1 µg, (2) 0.050 µg, (3) 0.020 µg, (4) 0.010 µg.