Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-OCRL antibody [EP10256] (ab181039)

Key features and details

  • Rabbit monoclonal [EP10256] to OCRL
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-OCRL antibody [EP10256]
    See all OCRL primary antibodies

  • Description

    Rabbit monoclonal [EP10256] to OCRL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, SH-SY5Y, HeLa and JAR cell lysates. Permeabilized SH-SY5Y cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as INPP5F

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP10256

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-OCRL antibody [EP10256] (ab181039)
    Western blot - Anti-OCRL antibody [EP10256] (ab181039)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: INPP5F knockout HAP1 cell lysate (20 µg)
    Lane 3: SH-SY5Y cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181039 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181039 was shown to recognize INPP5F when INPP5F knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP5F knockout samples were subjected to SDS-PAGE. ab181039 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-OCRL antibody [EP10256] (ab181039)
    Western blot - Anti-OCRL antibody [EP10256] (ab181039)
    All lanes : Anti-OCRL antibody [EP10256] (ab181039) at 1000 µg

    Lane 1 : 293T cell lysates
    Lane 2 : SH-SY5Y cell lysates
    Lane 3 : HeLa cell lysates
    Lane 4 : JAR cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 104 kDa

  • Flow Cytometry - Anti-OCRL antibody [EP10256] (ab181039)
    Flow Cytometry - Anti-OCRL antibody [EP10256] (ab181039)

    Flow cytometric analysis of permeabilized SH-SY5Y cells using ab181039 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-OCRL antibody [EP10256] (ab181039)

© 2021 Astana Biomed Group LLP. All rights reserved.