Recombinant Human IL1 Receptor I/IL-1R-1 protein (Fc Chimera) (ab83575)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human IL1 Receptor I/IL-1R-1 protein (Fc Chimera)
See all IL1 Receptor I/IL-1R-1 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical Sequence: LEADKCKEREEKIILVSSANEIDVRPCPLNPNEHKGTIT WYKDDSKTP VSTEQASRIHQHKEKLWFVPAKVEDSGHYYCVVRNSSYC LRIKISAKF VENEPNLCYNAQAIFKQKLPVAGDGGLVCPYMEFFKNEN NELPKLQWY KDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASY TYLGKQYPI TRVIEFITLEENKPTRPVIVSPANETMEVDLGSQIQLIC NVTGQLSDI AYWKWNGSVIDEDDPVLGEDYYSVENPANKRRSTLITVL NISEIESRF YKHPFTCFAKNTHGIDAAYIQLIYPVTNRSSNTKVDKKV EPKSCDKTH TCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVV VDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQ DWLNGKEYK CRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELT KNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY SKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
Amino acids
18 to 333 -
Additional sequence information
A DNA sequence encoding the signal peptide and extracellular domain of human IL-1RI (aa 1-333) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.
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Preparation and Storage
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Alternative names
- Antigen CD121a
- CD121 antigen-like family member A
- CD121a
see all -
Function
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant Human IL1 Receptor I/IL-1R-1 protein (Fc Chimera) (ab83575)Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
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SDS-PAGE - Recombinant Human IL1 Receptor I/IL-1R-1 protein (Fc Chimera) (ab83575)1D SDS-PAGE of ab83575 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 – MW markers; Lane 2 – ab83575; Lane 3 – ab83575 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83575 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 μg protein loaded per lane.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
