Mouse IL-1RA ELISA Kit (ab238258)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.3 pg/well
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-1RA ELISA Kit
See all IL-1RA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 2.6% Inter-assay Sample n Mean SD CV% Supernatant 3 3.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.3 pg/well -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103 99% - 108% Cell culture media 116 114% - 120% Hep Plasma 121 112% - 126% EDTA Plasma 96 93% - 98% Cit plasma 100 95% - 106% -
Assay time
1h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-1RA ELISA Kit (ab238258) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1RA protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-1RA with 10.3 pg/well sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-1RA Capture Antibody 1 x 600µl 10X Mouse IL-1RA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IL-1RA Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. -
Tissue specificity
The intracellular form of IL1RN is predominantly expressed in epithelial cells. -
Involvement in disease
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency -
Cellular localization
Cytoplasm and Secreted. - Information by UniProt
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Alternative names
- DIRA
- F630041P17Rik
- ICIL 1RA
see all -
Database links
- Entrez Gene: 16181 Mouse
- SwissProt: P25085 Mouse
- Unigene: 882 Mouse
Images
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Other - Mouse IL-1RA ELISA Kit (ab238258)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IL1RA standard curve in Sample Diluent NS.The IL1RA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IL1RA in mouse cell culture supernatant samples.The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: J774A.1 unstimulated 100%, and J774A.1 stimulated 12.5% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 426 pg/mL in unstimulated J774A.1 and 8,581 pg/mL in stimulated J774A.1 cell culture supernatant.
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Interpolated concentrations of native IL1RA in mouse serum and plasma samples.The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25% plasma (citrate) and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 200 pg/mL in serum, 216 pg/mL in plasma (citrate) and 488 pg/mL in plasma (heparin). -
Sandwich ELISA - Mouse IL-1RA ELISA Kit (ab238258)To learn more about the advantages of recombinant antibodies see here.
