Anti-ZAP70 (phospho Y315) antibody (ab60970)
Key features and details
- Rabbit polyclonal to ZAP70 (phospho Y315)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZAP70 (phospho Y315) antibody
See all ZAP70 primary antibodies -
Description
Rabbit polyclonal to ZAP70 (phospho Y315) -
Host species
Rabbit -
Specificity
Detects endogenous levels of ZAP70 only when phosphorylated at tyrosine 315. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 315 (S-V-YP-E-S).
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Positive control
- Lymph node tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 (phospho Y315) antibody (ab60970)ab60970 at 1/50 - 1/100 dilution staining ZAP70 in human lymph node by immunohistochemistry on paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
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Western blot - Anti-ZAP70 (phospho Y315) antibody (ab60970)All lanes : Anti-ZAP70 (phospho Y315) antibody (ab60970) at 500 cells
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) treated with 40 nM Ca2+, whole cell lysate
Lane 2 : Jurkat incubated with 40 nM Ca2+, whole cell lysate incubated with phospho-peptide
Predicted band size: 69 kDa
