Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit delta
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Hemoglobin subunit delta antibody [EPR8322(B)]
See all Hemoglobin subunit delta primary antibodies -
Description
Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit delta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Hemoglobin subunit delta aa 1-100. The exact sequence is proprietary.
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Positive control
- Human fetal heart, fetal liver, and red blood cell (RBC) lysates; Human endometrium and spleen tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8322(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human red blood cell (RBC) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
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Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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