Anti-Histone H4 (mono methyl R3) antibody (ab17339)
Key features and details
- Rabbit polyclonal to Histone H4 (mono methyl R3)
- Suitable for: WB, ICC/IF, PepArr
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (mono methyl R3) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (mono methyl R3) -
Host species
Rabbit -
Specificity
ab17339 detects a 14 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab17339 binds strongly to the Histone H4 mono methyl R3 peptide. -
Tested applications
Suitable for: WB, ICC/IF, PepArrmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Synthetic peptide within Human Histone H4 aa 1-100 (N terminal) (mono methyl R3) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab17770)
Images
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Western blot - Anti-Histone H4 (mono methyl R3) antibody (ab17339)Anti-Histone H4 (mono methyl R3) antibody (ab17339) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
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Peptide Array - Anti-Histone H4 (mono methyl R3) antibody (ab17339)All batches of ab17339 are tested in Peptide Array against peptides to different Histone H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - mono methyl R3 peptide (ab17770), indicating that this antibody specifically recognises the Histone H4 - mono methyl R3 modification.
ab17770 - Histone H4 - mono methyl R3
ab15821 - Histone H4 - unmodified
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl R3) antibody (ab17339)ICC/IF image of ab17339 stained human HepG2 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17339, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
