Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22229-5] to CD134 / OX40L receptor
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CD134 / OX40L receptor antibody [EPR22229-5]
    See all CD134 / OX40L receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR22229-5] to CD134 / OX40L receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    IP
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat splenocytes treated with 2.5ug/ml concanavalin A for 72h lysates. IHC-P: Mouse and rat spleen tissues. Flow Cyt: Rat and mouse primary splenocytes treated with 2.5µg/ml concanavalin A for 72h. IP: Rat splenocytes treated with 2.5µg/ml concanavalin A for 72h lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in rat spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.

    The section was incubated with ab229021 for 10 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.

    The section was incubated with ab229021 for 10 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

    CD134 / OX40L was immunoprecipitated from 0.35 mg rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate using ab229021 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab229021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at a 1/5000 dilution.

    Lane 1: Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate 10 μg (input)
    Lane 2: ab229021 IP in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229021 in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 20 seconds.

  • Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

    Flow cytometric analysis of mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/2000 dilution.

    Data shown were gated on viable cells.

  • Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

    Flow cytometric analysis of rat primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/2000 dilution.

    Data shown were gated on viable cells.

  • Western blot - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    Western blot - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    All lanes : Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021) at 1/1000 dilution

    Lane 1 : Untreated mouse splenocytes lysate
    Lane 2 : Mouse splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate
    Lane 3 : Untreated rat splenocytes lysate
    Lane 4 : Rat splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    CD134 / OX40L is a glycosylated protein and its expression is increased after concanavalin A treatment. PMID 23897980; PMID 17609274

  • Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
    Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

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