Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22229-5] to CD134 / OX40L receptor
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat

Product name

Anti-CD134 / OX40L receptor antibody [EPR22229-5]
See all CD134 / OX40L receptor primary antibodies
Description

Rabbit monoclonal [EPR22229-5] to CD134 / OX40L receptor
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Rat
IHC-P	Mouse Rat
IP	Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat splenocytes treated with 2.5ug/ml concanavalin A for 72h lysates. IHC-P: Mouse and rat spleen tissues. Flow Cyt: Rat and mouse primary splenocytes treated with 2.5µg/ml concanavalin A for 72h. IP: Rat splenocytes treated with 2.5µg/ml concanavalin A for 72h lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22229-5
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in rat spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.

The section was incubated with ab229021 for 10 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.

The section was incubated with ab229021 for 10 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

CD134 / OX40L was immunoprecipitated from 0.35 mg rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate using ab229021 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab229021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at a 1/5000 dilution.

Lane 1: Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate 10 μg (input)
Lane 2: ab229021 IP in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229021 in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds.
Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

Flow cytometric analysis of mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at 1/2000 dilution.

Data shown were gated on viable cells.
Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

Flow cytometric analysis of rat primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor^® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at 1/2000 dilution.

Data shown were gated on viable cells.
Western blot - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

All lanes : Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021) at 1/1000 dilution

Lane 1 : Untreated mouse splenocytes lysate
Lane 2 : Mouse splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate
Lane 3 : Untreated rat splenocytes lysate
Lane 4 : Rat splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

CD134 / OX40L is a glycosylated protein and its expression is increased after concanavalin A treatment. PMID 23897980; PMID 17609274
Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)

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