Anti-ARID3A antibody - C-terminal (ab227274)
Key features and details
- Rabbit polyclonal to ARID3A - C-terminal
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARID3A antibody - C-terminal
See all ARID3A primary antibodies -
Description
Rabbit polyclonal to ARID3A - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment within Human ARID3A (C terminal). The exact sequence is proprietary.
Database link: Q99856 -
Positive control
- WB:HCT 116 whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Mouse lymph node and testis tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARID3A antibody - C-terminal (ab227274)Anti-ARID3A antibody - C-terminal (ab227274) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 63 kDa7.5% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID3A antibody - C-terminal (ab227274)Paraffin-embedded mouse testis tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ARID3A antibody - C-terminal (ab227274)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARID3A (green) using ab227274 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining (lower panel).
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Immunoprecipitation - Anti-ARID3A antibody - C-terminal (ab227274)ARID3A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2 μg ab227274. Western blot was performed from the immunoprecipitate using ab227274 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate 40 μg (Input).
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Lane 3: ab227274 IP in HEK-293T whole cell lysate.
7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID3A antibody - C-terminal (ab227274)Paraffin-embedded mouse lymph node tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.
