Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Thursday March 04, 2021

Anti-CD69 antibody [EPR21811] (ab233395)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21811] to CD69
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-CD69 antibody [EPR21811]
See all CD69 primary antibodies
Description

Rabbit monoclonal [EPR21811] to CD69
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi treated with 50ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours whole cell lysate; H9 whole cell lysate. IHC-P: Human tonsil and cervix cancer tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21811
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD69 antibody [EPR21811] (ab233395)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD69 with ab233395 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in lymphoid cells of human tonsil (PMID: 2807372). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD69 antibody [EPR21811] (ab233395)

All lanes : Anti-CD69 antibody [EPR21811] (ab233395) at 1/1000 dilution

Lane 1 : Un-treated Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Daudi, treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 23 kDa
Observed band size: 28,32 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

PMA treatment increases the basal level of p28/32 in Daudi cells (PMID: 1617156).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD69 antibody [EPR21811] (ab233395)

Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling CD69 with ab233395 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in infiltrated lymphoid cells of human cervical cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD69 antibody [EPR21811] (ab233395)

Anti-CD69 antibody [EPR21811] (ab233395) at 1/1000 dilution + H9 (human cutaneous T lymphocyte lymphoma cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 23 kDa
Observed band size: 24,27 kDa why is the actual band size different from the predicted?

Exposure time: 6 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

A distinct band with an apparent molecular mass of 24 kDa and a fainter band with an apparent molecular mass of 27kDa were observed (PMID: 2784767).
Anti-CD69 antibody [EPR21811] (ab233395)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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