Human CD38 ELISA Kit (ab267655)
Key features and details
- Sensitivity: 0.41 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD38 ELISA Kit
See all CD38 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.41 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 133 124% - 143% Plasma 89 71% - 125% Cell culture media 73 70% - 80% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD38 ELISA Kit is designed for the quantitative determination of CD38 in plasma, serum and cell culture supernatant samples.
Plasma should be collected using EDTA and citrate as anti-coagulant. Heparin is NOT recommended.
Δ Note: Human CD38 concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for human CD38 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD38 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD38 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD38 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human CD38 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human CD38 Detection Antibody 2 vials Human CD38 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Acute lymphoblastic leukemia cells antigen CD38
- ADP ribosyl cyclase
- ADP ribosyl cyclase 1
see all -
Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
Images
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Standard curve
Human CD38 ELISA kit (ab267655).
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.