Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL25] to LAG-3 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LAG-3 antibody [CAL25] - BSA and Azide free
    See all LAG-3 primary antibodies

  • Description

    Rabbit monoclonal [CAL25] to LAG-3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IP, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow: Human peripheral blood mononuclear cell (PBMC) treated with 10µg/ml PHA for 48h. IP:HEK-293T transfected with GFP-tagged LAG-3 expression vector whole cell lysate.

  • General notes

    ab251604 is the carrier-free version of ab237718 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251604 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Lymphocyte Activation Gene 3.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LAG-3 using ab237718 at 1/800 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil is observed. The section was incubated with ab237718 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237718).

  • Flow Cytometry - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)
    Flow Cytometry - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)

    Flow cytometric analysis of 2% paraformaldehyde-fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cells (PBMC) treated with 10 µg/ml PHA for 48h labeling LAG-3 with ab237718 at 1/50 dilution (Right) compared to a Rabbit monoclonal IgG (ab172730, Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) was used as the secondary at 1/5000 dilution.
    Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining rabbit IgG (Left) or ab237718 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237718).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab237718 at 0.3 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237718).

  • Immunoprecipitation - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)
    Immunoprecipitation - Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)

    LAG-3 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged LAG-3 expression vector whole cell lysate using ab237718 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab237718 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HEK-293T transfected with GFP-tagged LAG-3 expression vector whole cell lysate 10 µg (input).
    Lane 2: ab237718 IP in HEK-293T GFP-tagged LAG-3 expression vector whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237718 in 293T transfected with GFP-tagged LAG-3 expression vector whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This antibody also detects a 16kDa cleaved fragment. (PMID: 15557174)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237718).

  • Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)
    Anti-LAG-3 antibody [CAL25] - BSA and Azide free (ab251604)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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