Human CD3 Epsilon ELISA Kit
(ab277708)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 38.1 pg/ml
- Range: 84 pg/ml - 5400 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 2.7% Inter-assay Sample n Mean SD CV% Extract 4 6.3% -
Sample type
Cell culture supernatant, Serum, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
38.1 pg/ml -
Range
84 pg/ml - 5400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 89 83% - 101% Serum 115 114% - 116% Cell Lysate 110 105% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD3 Epsilon ELISA kit (ab277708) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD3 Epsilon in human serum, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD3 Epsilon with 38.1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
T-Cell Surface Glycoprotein CD3 epsilon chain (CD3e), also known as T-cell surface antigen T3/Leu-4 epsilon is a type I membrane protein encoded by the CD3E gene. CD3e forms the T-cell receptor (TCR)-CD3 complex on T-lymphocyte cell surfaces by forming two heterodimers CD3E/CD3D (delta) and CD3E/CD3G (gamma). This complex plays a role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3e contains an immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic domain. When engaged by TCR, these motifs are phosphorylated by Src protein family kinases LCK and FYN, which activate downstream signaling pathways.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD3 epsilon Capture Antibody 1 x 600µl 10X Human CD3 epsilon Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CD3 epsilon Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD3 epsilon
- CD3e
- CD3e antigen
see all -
Database links
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD3 epsilon standard curve in Sample Diluent NS.The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human CD3 epsilon standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.The concentrations of CD3 epsilon were measured in duplicate and interpolated from the CD3 epsilon standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.