Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269)
![Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269)](https://www.abcam.com/ps/products/133/ab133269/Images/ab133269-146714-anti-lamin-a-c-antibody-epr4520-11-western-blot.jpg "Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4520-11] to Cleaved Lamin A + C (Asp230)
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11]
See all Cleaved Lamin A + C (Asp230) primary antibodies -
Description
Rabbit monoclonal [EPR4520-11] to Cleaved Lamin A + C (Asp230) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
corresponding to Human Cleaved Lamin A + C (Asp230) aa 200-300.
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Positive control
- Lysate of NIH/3T3 cells treated with staurosporine, NIH/3T3 cell lysate, lysate of HeLa cells treated with camptothecine, HeLa whole cell lysate (ab150035)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4520-11 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269)All lanes : Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269) at 1/50000 dilution
Lane 1 : Lysate of NIH 3T3 cells treated with staurosporine
Lane 2 : NIH 3T3 cell lysate
Lane 3 : Lysate of HeLa cells treated with camptothecine
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?
Note: The observed bands are possible cleavage fragments. -
![Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269)](https://www.abcam.com/ps/products/133/ab133269/Images/ab133269-2-benefits-of-recombinant-antibodies.png)
Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269)
