Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL77] to LAG-3 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LAG-3 antibody [CAL77] - BSA and Azide free
    See all LAG-3 primary antibodies

  • Description

    Rabbit monoclonal [CAL77] to LAG-3 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC-P is only recommended for human samples. We obtained negative staining results with mouse.

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Human PBMC. Mouse splenocytes treated with ConA.

  • General notes

    ab251606 is the carrier-free version of ab237720 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251606 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Lymphocyte Activation Gene 3.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LAG-3 with ab237720 at 1/900 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil is observed. The section was incubated with ab237720 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 30 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 with ab237720 at 0.25 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237720).

  • Flow Cytometry - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)
    Flow Cytometry - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml PHA for 2 days, labeling Lymphocyte Activation Gene 3 with ab237720 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237720 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237720).

  • Flow Cytometry - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)
    Flow Cytometry - Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes treated with 2.5μg/ml Concanavalin A (ConA) for 3 days, labeling LAG-3 with ab237720 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237720 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237720).

  • Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)
    Anti-LAG-3 antibody [CAL77] - BSA and Azide free (ab251606)

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