Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free
See all BCL2L15 primary antibodies
Description

Rabbit monoclonal [EPR17873] to BCL2L15 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human colon and gastric adenocarcinoma tissues. ICC/IF: HEK-293T cells transfected with GFP-BCL2L15 expression construct; HEK-293T cells transfected with BCL2L15 expression vector. Flow Cyt: HEK-293T cells transfected with BCL2L15 (GFP-Tag).
General notes
ab271949 is the carrier-free version of ab184932. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17873
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) cells, untransfected or transfected with with BCL2L15 expression vector, labeling BCL2L15 with ab184932 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with BCL2L15 expression vector.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and AlexaFluor^® 594 Goat anti-Mouse (ab150120) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab184932 at 1/500 dilution followed by AlexaFluor^® 594 Goat anti-Mouse (ab150120) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184932).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on human colon epithelium is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184932).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasm staining on the human gastric adenocarcinoma is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184932).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: no staining on human liver.

Counter stained with hematoxylin.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184932).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: no staining on human endometrium.

Counter stained with hematoxylin.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184932).
Immunocytochemistry/ Immunofluorescence - Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney) cells transfected with GFP-BCL2L15 expression construct, labeling BCL2L15 with ab184932 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/500 dilution (red). Confocal image showing cytoplasmic and weak nuclear staining on HEK293T transfected with GFP-BCL2L15 expression construct.
The nuclear counter stain is DAPI (blue). GFP is detected with an anti-GFP antibody (green) at 1/500 dilution.
-ve control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184932).
Flow Cytometry - Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)

Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with BCL2L15 (GFP-Tag) labeling BCL2L15 with ab184932 at 1/300 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG (PE) at 1/150 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184932).
Anti-BCL2L15 antibody [EPR17873] - BSA and Azide free (ab271949)