Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCL2L15 antibody [EPR17873] (ab184932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17873] to BCL2L15
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BCL2L15 antibody [EPR17873]
    See all BCL2L15 primary antibodies

  • Description

    Rabbit monoclonal [EPR17873] to BCL2L15

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with a GFP-tagged BCL2L15 expression vector whole cell lysate. IHC-P: Human colon and gastric adenocarcinoma tissues. ICC/IF: HEK-293T cells transfected with GFP-BCL2L15 expression construct; HEK-293T cells transfected with BCL2L15 expression vector. Flow Cyt: HEK-293T cells transfected with BCL2L15 (GFP-Tag).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17873

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Western blot - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    All lanes : Anti-BCL2L15 antibody [EPR17873] (ab184932) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) non-transfected whole cell lysate
    Lane 2 : HEK-293T transfected with a GFP-tagged BCL2L15 expression vector whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular mass of 45 kDa includes the 18 kDa BCL2L15 peptide and the 27 kDa GFP tag.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on human colon epithelium is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)

    Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasm staining on the human gastric adenocarcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human liver.

    Counter stained with hematoxylin.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human endometrium.

    Counter stained with hematoxylin.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Immunocytochemistry/ Immunofluorescence - Anti-BCL2L15 antibody [EPR17873] (ab184932)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney) cells transfected with GFP-BCL2L15 expression construct, labeling BCL2L15 with ab184932 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red). Confocal image showing cytoplasmic and weak nuclear staining on HEK293T transfected with GFP-BCL2L15 expression construct.

    The nuclear counter stain is DAPI (blue). GFP is detected with an anti-GFP antibody (green) at 1/500 dilution.

    -ve control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Immunocytochemistry/ Immunofluorescence - Anti-BCL2L15 antibody [EPR17873] (ab184932)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) cells, untransfected or transfected with with BCL2L15 expression vector, labeling BCL2L15 with ab184932 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with BCL2L15 expression vector.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and AlexaFluor® 594 Goat anti-Mouse (ab150120) at 1/500 dilution (red).

    The negative controls are as follows;
    -ve control 1: ab184932 at 1/500 dilution followed by AlexaFluor® 594 Goat anti-Mouse (ab150120) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.

  • Flow Cytometry - Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Flow Cytometry - Anti-BCL2L15 antibody [EPR17873] (ab184932)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with BCL2L15 (GFP-Tag) labeling BCL2L15 with ab184932 at 1/300 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG (PE) at 1/150 dilution was used as the secondary antibody.

  • Anti-BCL2L15 antibody [EPR17873] (ab184932)
    Anti-BCL2L15 antibody [EPR17873] (ab184932)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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