Anti-BCL2L15 antibody [EPR17873] (ab184932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17873] to BCL2L15
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-BCL2L15 antibody [EPR17873]
See all BCL2L15 primary antibodies -
Description
Rabbit monoclonal [EPR17873] to BCL2L15 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with a GFP-tagged BCL2L15 expression vector whole cell lysate. IHC-P: Human colon and gastric adenocarcinoma tissues. ICC/IF: HEK-293T cells transfected with GFP-BCL2L15 expression construct; HEK-293T cells transfected with BCL2L15 expression vector. Flow Cyt: HEK-293T cells transfected with BCL2L15 (GFP-Tag).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17873 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-BCL2L15 antibody [EPR17873] (ab184932) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) non-transfected whole cell lysate
Lane 2 : HEK-293T transfected with a GFP-tagged BCL2L15 expression vector whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular mass of 45 kDa includes the 18 kDa BCL2L15 peptide and the 27 kDa GFP tag.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on human colon epithelium is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasm staining on the human gastric adenocarcinoma is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: no staining on human liver.
Counter stained with hematoxylin.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L15 antibody [EPR17873] (ab184932)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling BCL2L15 with ab184932 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: no staining on human endometrium.
Counter stained with hematoxylin.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney) cells transfected with GFP-BCL2L15 expression construct, labeling BCL2L15 with ab184932 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red). Confocal image showing cytoplasmic and weak nuclear staining on HEK293T transfected with GFP-BCL2L15 expression construct.
The nuclear counter stain is DAPI (blue). GFP is detected with an anti-GFP antibody (green) at 1/500 dilution.
-ve control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) cells, untransfected or transfected with with BCL2L15 expression vector, labeling BCL2L15 with ab184932 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with BCL2L15 expression vector.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and AlexaFluor® 594 Goat anti-Mouse (ab150120) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab184932 at 1/500 dilution followed by AlexaFluor® 594 Goat anti-Mouse (ab150120) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution. -
Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with BCL2L15 (GFP-Tag) labeling BCL2L15 with ab184932 at 1/300 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG (PE) at 1/150 dilution was used as the secondary antibody.
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