Anti-GRP78 BiP antibody [3C5-1A4] (ab212054)
![Anti-GRP78 BiP antibody [3C5-1A4] (ab212054)](https://www.abcam.com/ps/products/212/ab212054/Images/ab212054-282648-anti-grp78-bip-antibody-3c5-1a4-immunofluorescence.jpg "Anti-GRP78 BiP antibody [3C5-1A4] (ab212054)")
Key features and details
- Mouse monoclonal [3C5-1A4] to GRP78 BiP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-GRP78 BiP antibody [3C5-1A4]
See all GRP78 BiP primary antibodies -
Description
Mouse monoclonal [3C5-1A4] to GRP78 BiP -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Rat GRP78 BiP.
Database link: P06761 -
Positive control
- ICC/IF: NIH/3T3 cells. WB: NIH/3T3 and HEK-293 cell lysate; Rat brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3C5-1A4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody [3C5-1A4] (ab212054)
4% formaldehyde-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells stained for GRP78 BiP (green) using ab212054 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse 488 at 1/100 for 60 minutes at RT. Counterstain: DAPI (blue) nuclear stain at 1/5000 for 5 minutes RT.
(A) DAPI nuclear stain (B) ab212054 (C) Merge.
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![Western blot - Anti-GRP78 BiP antibody [3C5-1A4] (ab212054)](https://www.abcam.com/ps/products/212/ab212054/Images/ab212054-282649-anti-grp78-bip-antibody-3c5-1a4-western-blot.jpg)
Western blot - Anti-GRP78 BiP antibody [3C5-1A4] (ab212054)All lanes : Anti-GRP78 BiP antibody [3C5-1A4] (ab212054) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : Rat brain lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Secondary
All lanes : HRP Goat Anti-Mouse at 1/50 dilution
Predicted band size: 72 kDaBlocking: 5% milk + TBST for 1 hour at RT.
Color Development: TMB solution for 5 minutes at RT.
