Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4040(2)] to GRP78 BiP
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-GRP78 BiP antibody [EPR4040(2)]
See all GRP78 BiP primary antibodies
Description

Rabbit monoclonal [EPR4040(2)] to GRP78 BiP
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, LnCaP, ECV-304, C6, NIH/3T3, PC-12, C6 (Rat glial tumor glial cell), RAW264.7, MCF7, 293T, U-87 MG, THP-1, MDA-MB-231 and HepG2 cell lysates; Human liver lysates; Mouse heart, lung, testis and liver lysates; Rat liver lysates. IHC-P: Human brain, ovarian cancer and testis tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4040(2)
Isotype

IgG
Research areas

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 8 : Human uterus lysates
Lane 9 : Human liver lysates
Lane 10 : Mouse heart lysates
Lane 11 : Mouse lung lysates
Lane 12 : Mouse testis lysates
Lane 13 : Mouse liver lysates
Lane 14 : Rat liver lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 72 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Exposure time: 37 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian cancer tissue sections labeling GRP78 BiP with Purified ab108613 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.
Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa treated with 2.5 µg/ml tunicamycin for 24h whole cell lysates
Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 4 : HUVEC treated with 10 µg/ml tunicamycin for 48h whole cell lysates
Lane 5 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 6 : Raw 264.7 treated with 5 µg/ml tunicamycin for 18h whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds
Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 0.1 µg/ml (purified) + C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 72 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

Immunohistochemical analysis of paraffin-embedded Human testis tissue using unpurified ab108613 at 1/250
Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution (purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 12 hours whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 48 hours whole cell lysates
Lane 5 : MEF (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 72 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938

Exposure time:
Lanes 1 and 2: 3 seconds
Lanes 3 and 4: 30 seconds
Lanes 5 and 6: 5 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using unpurified ab108613 at 1/250
Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution (Unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : ECV-304 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution (Unpurified)

Lane 1 : C6 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : RAW264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

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