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Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14972] to CYP1B1 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CYP1B1 antibody [EPR14972] - C-terminal
    See all CYP1B1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14972] to CYP1B1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human renal adenocarcinoma tissue. WB: Human breast cancer, fetal kidney and fetal brain lysate; Rat brain and spleen lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    All lanes : Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/20000 dilution

    Lane 1 : Human breast cancer lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)

    Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CYP1B1 with ab185954 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    All lanes : Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa

  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa

  • Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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