Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

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Anti-Histone H3 (mono+di+tri methyl K14) antibody [EPR17708-253] (ab177220)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17708-253] to Histone H3 (mono+di+tri methyl K14)
Suitable for: PepArr, Dot blot, ICC/IF, WB
Reacts with: Mouse, Human

Product name

Anti-Histone H3 (mono+di+tri methyl K14) antibody [EPR17708-253]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR17708-253] to Histone H3 (mono+di+tri methyl K14)
Host species

Rabbit

Tested Applications & Species

Application	Species
Dot	Mouse
ICC/IF	Human
PepArr	Human
WB	Mouse Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NIH/3T3 and HeLa whole cell lysates. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17708-253
Isotype

IgG
Research areas

Western blot - Anti-Histone H3 (mono+di+tri methyl K14) antibody [EPR17708-253] (ab177220)

All lanes : Anti-Histone H3 (mono+di+tri methyl K14) antibody [EPR17708-253] (ab177220) at 1/1000 dilution

Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% BSA/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono+di+tri methyl K14) antibody [EPR17708-253] (ab177220)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (mono+di+tri methyl K14) with ab177220 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab177220 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Dot Blot - Anti-Histone H3 (mono+di+tri methyl K14) antibody [EPR17708-253] (ab177220)

Dot blot analysis of Histone H3 (mono methyl K14) peptide (Lane 1), Histone H3 (di methyl K14) (Lane 2), Histone H3 (tri methyl K14) peptide (Lane 3), and Histone H3 unmodified peptide (Lane 4), labeled using ab177220 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/2500 dilution.
Blocking/Dilution buffer: 5% BSA/TBST.
Exposure time: 3 minutes.
Peptide Array - Anti-Histone H3 (mono+di+tri methyl K14) antibody [EPR17708-253] (ab177220)

ab177220 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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