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Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)

Price and availability

348 441 ₸

Availability

Order now and get it on Thursday March 04, 2021

Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [E379] to NF-kB p65
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

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Overview

  • Product name

    Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379]
    See all NF-kB p65 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [E379] to NF-kB p65
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Alternative versions available:
    Anti-NF-kB p65 antibody [E379] (ab32536) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E379
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • RelA (p65)
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response
    • Metabolism
    • Types of disease
    • Obesity
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • RelA (p65)

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)

    ab190205 staining NF-kB p65 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab190205 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at a dilution of 1 in 250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    This product also gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)
    Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)

  •  
  • Product image

    Anti-NF-kB p65 (phospho S529) antibody (ab84333)

    Applications: WB

  •  
  • Product image

    Anti-NF-kB p65 (phospho S311) antibody (ab194926)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-NF-kB p65 (phospho S468) antibody (ab194921)

    Applications: ICC/IF, WB

  •  
  • Product image

    Alexa Fluor® 555 Anti-NF-kB p65 antibody [E379] (ab214634)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-NF-kB p65 antibody [E379] (ab32536)

    Applications: ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-NF-kB p65 (phospho S536) antibody (ab28856)

    Applications: IHC-P, WB

  •  
  • Product image

    Biotin Anti-NF-kB p65 antibody [E379] (ab205823)

    Applications: IHC-P

  •  
  • Product image

    Anti-NF-kB p65 (phospho S529) antibody [EPR2379] (ab109458)

    Applications: Flow Cyt, IP, WB

  •  
  • Product image

    Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533)

    Applications: ChIP, Dot, WB

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