Anti-NF-kB p65 (phospho S536) antibody (ab86299)
Key features and details
- Rabbit polyclonal to NF-kB p65 (phospho S536)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NF-kB p65 (phospho S536) antibody
See all NF-kB p65 primary antibodies -
Description
Rabbit polyclonal to NF-kB p65 (phospho S536) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human NF-kB p65. around the phosphorylation site of Serine 536.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab86299 was affinity purified using an epitope specific to Phospho NFkB p65 (S536) immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S536) antibody (ab86299) Kiss T et al., PLoS ONE. 2014;9(8):e104890. Fig #3B.; doi:10.1371/journal.pone.0104890. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labeling NF-kB p65 (phospho S536) with ab86299 at 1/20 dilution. Tissue sections were fixed in 6%formalin and embedded into paraffin, 5 µm thin sections were cut with a microtome. Sections were stained with haematoxylin–eosin. Slides were deparaffinized in xilene, rehydrated in graded ethanol series, and washed in distilled water. Heat induced epitope retrieval was performed by boiling the tissue sections in citrate buffer in a microwave oven at 750 W followed by cooling at room temperature for 20 minutes. Slides were washed in tris buffered saline (TBS) solution (pH = 7,6) followed by blocking of endogenous peroxidase for 10 minutes at room temperature. Slides were washed in TBS. Nonspecific sites were blocked for 10 minutes at room temperature. Without washing, the primary antibody ab86299 was applied. Incubation with the primary antibody was performed for 1 hour at room temperature followed by washing in TBS. Anti-rabbit secondary antibody was applied for 30 minutes at room temperature followed by repeated washing in TBS.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S536) antibody (ab86299)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling NF-kB p65 (phospho S536) with ab86299 at 1/200 (1µg/ml) and 1/1000 (0.2µg/ml). Detection: DAB. -
Western blot - Anti-NF-kB p65 (phospho S536) antibody (ab86299)All lanes : Anti-NF-kB p65 (phospho S536) antibody (ab86299) at 0.04 µg/ml
Lane 1 : Jurkat whole cell lysate treated with TNF alpha and
Calyculin A
Lane 2 : Jurkat whole cell lysate mock treated
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S536) antibody (ab86299)IHC image of NF-kB p65 (phospho S536) staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86299, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
